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. 2019 Jan 9;30(1):88–105. doi: 10.1089/hum.2018.028

Figure 1.

Figure 1.

Adeno-associated virus serotype 5 (AAV-5)-green fluorescent protein (GFP) transfection of the cochlea. Three concentrations of virus were studied: undiluted, 1:10 dilution, and 1:20 dilution. As expected, 1 μL of the undiluted virus resulted in the highest level of inner hair cells (IHC), outer hair cells (OHC), and spiral ganglion neurons (SGN) cell transfection. (A) Two weeks following transfection, numerous transfected IHCs (white arrows) and a few OHCs (red arrows) in addition to some pillar and supporting cells strongly express GFP. (B) Higher magnification of the transfected IHC (white arrows) in the same mouse cochlea as shown in (A). (C) Mid-modiolar section from the injected left cochlea demonstrates transfection of the IHC (arrow). Further, most of the SGN in the field also strongly express GFP. (D) Mid-modiolar section of the contralateral, untreated right cochlea, showing no GFP expression in IHC, OHC, or SGN.