Figure 3.

hGDNF overexpression causes damage to the cerebellar Purkinje cells. Immunofluorescence staining of brain sections using anti-calbindin (a protein marker of the Purkinje cells) revealed strong labeling of the cerebellar Purkinje cell somata in the control, and loss of the Purkinje cells in the undiluted AAV5-hGDNF transfection group. This loss of Purkinje cells may explain some of the neurologic symptoms observed (tremors, poor coordination, and ataxia). White asterisks indicate the approximate locations of missing Purkinje cells, and red arrows show abnormal Purkinje cells in the treated mice.