Figure 2.

LppZ recruits innate cells and induces pro-inflammatory cytokine production in the murine air pouch model. After injecting CMC (vehicle), LppZ (LppZ) or Ag85A (Ag85A) recombinant protein in the pouches, mice were sacrificed at 4 h and 24 h. Cells in the pouches were collected. (A) Total cell numbers recruited in the pouches. (B) Representative graphs showing the gating strategy for analysis of infiltrating cell subsets. The absolute numbers of CD11b⁺ cells (C), CD11b⁺Ly6G^hiF4/80⁻ neutrophils (D) and CD11b⁺Ly6G⁻F4/80⁺ macrophages (E) were analyzed by flow cytometry. The levels of IL-1β (F), IL-6 (G), TNF-α (H), and myeloperoxidase (MPO) (I) were measured in the exudates (n = 3–6 mice per group). *p < 0.05, **p < 0.01, ***p < 0.001.