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. 2019 Jan 16;9:3190. doi: 10.3389/fimmu.2018.03190

Figure 3.

Figure 3

LppZ evokes increased IFN-γ and TNF-α release from splenocytes of immunized mice. (A) C57BL/6 female mice (6–8 weeks old) were immunized with LppZ (LppZ/ADJ) or Ag85A (Ag85A/ADJ) recombinant proteins emulsified with adjuvant, or adjuvant only (ADJ). Splenocytes from different immunization group were collected. For ex vivo stimulation, 2.5 × 105 cells were incubated with LppZ (10 μg/mL) or Ag85A (10 μg/mL) for 20 h. Antigen-specific IFN-γ-producing cells were measured by ELISpot assay. ConA was treated as a positive control. (B,C) 5 × 105 cells were incubated with LppZ (10 μg/mL) for 20 h. Antigen-specific IFN-γ, TNF-α, and IL-2 producing CD4+ (B) or CD8+ (C) T cells from the ADJ or LppZ/ADJ group were measured by flow cytometry. PMA/Ionomycin was a positive control and GST was an irrelevant control. UNS: without ex vivo antigen stimulation. *p < 0.05, **p < 0.01, ***p < 0.001.