Figure 6.

M.tb antigen-specific Th1-type cytokine production in CD4⁺ or CD8⁺ T cells from the lungs of virulent H37Rv-challenged mice. Four weeks after bacteria challenge, mice were sacrificed and lymphocytes from the lungs were collected. For ex vivo stimulation, 5 × 10⁵ cells from different groups (ADJ, LppZ/ADJ or Ag85A/ADJ) were incubated with 10 μg/mL LppZ, Ag85A or PPD respectively, for 20 h. Intracellular cytokine profile for IFN-γ, TNF-α and IL-2 in individual cells from different groups were measured by flow cytometry of CD4⁺ (A) or CD8⁺ (B) T cells.