Figure 7.

Polyfunctional CD4⁺ and CD8⁺ T cell distribution in the lungs of the immunized mice after H37Rv challenge. Pulmonary lymphocytes from different groups (ADJ, LppZ/ADJ or Ag85A/ADJ) were collected and stimulated ex vivo with LppZ (A,D), Ag85A (B,E) or PPD (C,F). Frequencies of antigen-specific CD4⁺ or CD8⁺ T cells producing single or multiple cytokines were analyzed by flow cytometry. Single or multiple cytokine production was shown on the x-axis of the bar chart. The percentages of T cells expressing single (1+), double (2+) or triple (3+) cytokines were represented as pie charts. *p < 0.05.