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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Mol Cancer Res. 2018 Aug 2;16(12):1991–2002. doi: 10.1158/1541-7786.MCR-18-0366

Figure 5.

Figure 5.

Role of PKA in TGFβ-NR4A1 interactions. (A) The Promega PKA activity assay kit was used to investigate PKA activation by TGFβ, MKK4(WT) and MKK7(CA) alone and in combination with various agents and by TGFβ plus MKK7(DN). A549 cells were treated with TGFβ or transfected with MKK7(CA) and MKK4(WT) alone or in combination with 14–22 Amide or cotransfected with siPKA-Cα (knockdown) and effects on cell migration (B and C) and intracellular location (nucleus vs. cytosol) of NR4A1 (D and E) were determined by Boyden chamber and western blot assays, respectively. The effects of 14–22 Amide (F) and siPKA-Cα (G) on the time-dependent expression of TGFβ-induced proteins was determined by western blot analysis of whole cell lysates. Significant (p<0.05) inhibition of induced migration (determined in triplicate) by 14–22 Amide or siPKA-Cα is indicated (*). The NR4A1 band relative to β-actin in western blots (D, E) was quantitated, and control values of NR4A1 were set to 1.0.