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. 2019 Jan 18;12:647–656. doi: 10.2147/OTT.S188691

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© 2019 Xu et al. This work is published and licensed by Dove Medical Press Limited

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Manipulation of LINC00662 expression regulates cell proliferation in OSCC cells.

Notes: (A) The expression levels of LINC00662 in different OSCC cells. (B) ISG15 cells were transfected with siLINC00662 and LINC00662 overexpression plasmids, and the expression of LINC00662 was measured by qRT-PCR. (C) Cell proliferation ability was detected using CCK-8 assay at 12, 24, 48, and 72 hours. (D) Cell growth viability was measured using EdU staining method at 48 hours. (E, F) Flow cytometry was used to analyze the cell cycle distribution in ISG15 cells. *P<0.05 vs control or NC group. **P<0.01 vs control or NC group. ***P<0.001 vs control or NC group.

Abbreviations: CCK-8, Cell Counting Kit-8; CTRL, control; NC, negative control; OSCC, oral squamous cell carcinoma; qRT-PCR, quantitative reverse-transcription polymerase chain reaction.