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. 2019 Jan 16;9:3278. doi: 10.3389/fmicb.2018.03278

FIGURE 1.

FIGURE 1

Integrin β1 mediated E. coli O157:H7 adhesion to Caco-2 cells. (A) mRNA expression of integrin β1. (B) Immunofluorescence staining for integrin β1. Blue, DAPI; Red, integrin β1. Images were taken at 200 × magnification. (C) Bacterial adhesion to Caco-2 cells with or without integrin β1 antibody blocking (Ab BLK). (D) Integrin β1 content and bacterial adhesion to Caco-2 cells transfected with scramble control (Con sgRNA) or integrin β1 knockout (ITGB1 sgRNA) plasmid. (E) Co-immunoprecipitation analysis of integrin β1 and FAK interaction. Input, the whole cell lysate detected with β-actin; IP, lysates post integrin β1 immunoprecipitation were detected with integrin β1 or FAK antibody. Caco-2 cells were co-cultured with EDL933 strain for 4 h before respective analyses. Means ± SEM; n = 4. ∗∗P ≤ 0.01.