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. 2019 Jan 16;9:3278. doi: 10.3389/fmicb.2018.03278

FIGURE 4.

FIGURE 4

Integrin β1 was associated with E. coli O157:H7-mediated actin polymerization. Immunofluorescence staining for F-actin in Caco-2 cells. Caco-2 cells were immuno-blocked with integrin β1 antibody (Ab BLK) or transfected with integrin β1 sgRNA (ITGB1 sgRNA) plasmid followed by infection with EDL933 strain for 4 h. Cells were then subjected to phalloidin-TRIC staining. Con, Caco-2 cells without infection; EDL933, Caco-2 cells infected with EDL933 strain. ITGB1 sgRNA+EDL933, integrin β1 knockout Caco-2 cells infected with EDL933 strain; Ab BLK+EDL933, integrin β1 blocked Caco-2 cells infected with EDL933 strain. Blue, DAPI; Red, F-actin; Images were taken at 200 × magnification.