FIGURE 7.

Quercetin reduced E. coli O157:H7 adhesion and prevented integrin β1 overexpression induced by E. coli O157:H7 infection. (A,B) mRNA expression and protein level of integrin β1. (C) Immunofluorescence staining of integrin β1. Red, integrin β1. Images were taken at 200 × magnification. (D) Adhesion of EDL933 to Caco-2 cells treated with or without quercetin. (E) Co-immunoprecipitation analysis of integrin β1 and FAK interaction. Input, the whole cell lysate detected with β-actin; IP, the immunoprecipitated lysates were detected with integrin β1 or FAK antibody. Caco-2 cells were pretreated with 0 or 200 μM quercetin for 12 h, then followed by infection with EDL933 strain for 4 h when cells were collected for respective assays. Means ± SEM; n = 4. ^∗∗P ≤ 0.01; ^∗P ≤ 0.05.