Table 2.
Characteristics of the top 10 antigenic targets associated with the highest antibody responses 30 days post-challenge in controlled human malaria infection participants.
| Gene ID | Description | Name | Allele | Locationa | AA position | Expression tag | Reference |
|---|---|---|---|---|---|---|---|
| PF3D7_0532100 | Early transcribed membrane protein 5 | Etramp 5 Ag 1 | 3D7 | iRBC/PVM | 26–111 | GST | Spielmann et al., 2003; Tetteh, unpublished |
| PF3D7_1035300 | Glutamate rich protein R2 | GLURP-R2 | F32 | Merozoite (Peripheral) | 705–1178 | Hisx6 | Theisen et al., 1995 |
| PF3D7_0207000 | Merozoite surface protein 4 | MSP4 | D10 | Merozoite surface (GPI-anchored) | 43–107 | GST | Marshall et al., 1997 |
| PF3D7_0930300 | 19 kDa fragment of the merozoite surface protein-1 | MSP1-19 | Wellcome | Merozoite surface (GPI-anchored) | 1631–1726 | GST | Burghaus and Holder, 1994 |
| PF3D7_0423700 | Early transcribed membrane protein 4 | Etramp 4 Ag 2 | 3D7 | iRBC/PVM | 76–136 | GST | Spielmann et al., 2003; Tetteh, unpublished |
| PF3D7_1035300 | Glutamate rich protein R0 | GLURP-R0 | F32 | Merozoite (Peripheral) | 94–489 | Hisx6 | Theisen et al., 1995 |
| PF3D7_0206900 | Merozoite surface protein 5 | MSP5 | 3D7 | Merozoite surface (GPI-anchored) | 147–207 | GST | Marshall et al., 1998 |
| PF3D7_1021800 | Schizont egress antigen 1 | SEA-1 | 3D7 | Schizont/Maurer’s cleft | 810–1083 | GST | Raj et al., 2014; Tetteh, unpublished |
| PF3D7_0304600 | Circumsporozoite protein | CSP | 3D7 | Sporozoite surface | 20–373 | n/a | Kastenmüller et al., 2013 |
| PF3D7_1133400 | Apical membrane antigen 1 | AMA1 | FVO | Sporozoite/Merozoite (Micronemes) | 97–546 | Hisx6 | Collins et al., 2007 |
GPI, glycosylphosphatidylinositol; PVM, parasitophorous vacuolar membrane; iRBC, infected red blood cell; GST, glutathione S-transferase; kDa, kilodalton; AA, amino acid. aLocation information is based on published literature and mass spec proteomic data (plasmodb).