Figure 5.

RPE melanosome ultrastructure in 14-day-old mutant mice. High magnification transmission electron micrographs showing the abnormal size and multispherical shapes of selected macromelanosomes from 14-day-old mutant RPE cells (A, B; n = 4). Still frame from a 3D tomographic analysis (see Supplementary Video 1) of a thick epon section from a 14-day-old mutant RPE cell (C). The multispherical shape of the macromelanosome is surrounded by a single limiting membrane encompassing the entire structure (highlighted in green) and is comprised of three separate melanosomes, each identifiable by the perimeters of individual packed melanin cores (highlighted in red [D]). Scale bars: A, B = 1 μm and C, D = 200 nm.