Paxillin phosphorylation label intensities within the ONH increase after ONT, but not when combined with IOP elevation. (A) ONH sections labeled with antiphosphorylated paxillin (p-paxillin) antibodies in control eyes and eyes exposed to CEI, ONT, or a combination of CEI and ONT. ONH p-paxillin label intensity significantly increased 1 day after CEI alone, relative to controls (A, B). However, when CEI and ONT were combined, ONH p-paxillin label intensity did not significantly change relative to controls or CEI alone (A, B). (B) Mean p-paxillin label intensities within the ONH of control eyes and eyes exposed to ONT only, CEI only, or combined CEI + ONT. Error bars indicate SEM; *P < 0.05 and indicates statistical significance; n = 6, 8, 6, 7, 7, and 4 for each column from left to right, and represents individual animals.