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. 2018 Sep 26;20(2):183–191. doi: 10.1080/15384047.2018.1523094

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© 2018 The Author(s). Published by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — Resveratrol promoted GJIC in CBRH7919 cells. (A) Resveratrol intensified the spread of Lucifer yellow from the wounded cells to the neighboring cells, as shown by the scrape loading/dye transfer assay. (B) Double-fluorescence assay showing the promotion of GJIC by resveratrol in CBRH7919 cells. Q1: recipient cells (calcein⁻ DiI⁺), Q4: donor cells (calcein⁺ DiI⁻), Q2: DiI- and calcein-positive cell populations indicating the extent of GJIC, Q3: calcein⁻ DiI⁻ cells. *P < 0.05 vs. control group. Calcein, transferable green dye, passes readily through gap junctions, whereas Dil as a lipophilic red fluorescent dye does not.