Figure 3.

Targeting miR-155 promoted the apoptosis and reduced the pro-lymphangiogenic capacity of NKTCLs. A. and B. SNK-6 or YTS cells were transfected with either miR-155 inhibitor or NC. At 48 h after the transfection, cellular apoptosis was examined by flow cytometry following dual staining with Annexin V and propidium iodide (PI). The representative flow images were shown in A and the quantification of % of AnnexinV⁺ apoptotic cells shown in B. C. CM was collected from cells treated with NC or miR-155 inhibitor and applied to human lymphatic endothelial cells (HLECs) transfected with siVEGFR3 or control siRNA (siNC). The lymphatic tube formation was imaged under light microscopy. D. The lymphangiogenesis was scored and compared between indicated groups. *P < 0.05; **P < 0.01; ***P < 0.001.