Figure 2.

Efficient depletion of CDK2 using IAA and Dox. (a) CDK2^KO:AID-CDK2 cells. CDK2^KO cells expressing AID-CDK2 were generated as described in Materials and Methods. After growing in the absence of IAA and Dox, individual clones were isolated. Lysates were prepared and the expression of CDK2 was detected with immunoblotting. Equal loading of lysates was confirmed by immunoblotting for actin. The positions of molecular size standards (in kDa) are indicated on the left. Control HeLa cells were included in the analysis to indicate the position and abundance of endogenous CDK2. (b) CDK2^KO cells expressing AID-CDK2 were grown in the presence of IAA and/or Dox and harvested at the indicated time. Lysates were prepared and the expression of CDK2 was detected using immunoblotting. Equal loading of lysates was confirmed by actin analysis.