Figure 5.

CENPF reduce metabolism in prostate cancer cells. (a) Western blot analysis of key signal transduction proteins. (b) Western blot analysis of phospho-tyrosine (pY) signaling was performed in CENPF^KO cells and Ctrl. (c) A workflow including phospho-tyrosine-enrichment by protein pull-down and LC–MS/MS illustrates the experimental design to identify the tyrosine phosphorylated proteins specifically enriched in CENPF^KO cells. (d) The 10 top proteins identified from B. (e) Representative MS spectrum of PKM2, ENO1, MHY9, and VIM, which were identified in this study. (f) Phosphorylation of PKM2 at Y¹⁰⁵ and non-phosphorylated form of PKM2 were assessed by western blot analysis in CENPF^KO cells and Ctrl. β-actin was used as the loading control.