Figure 6.

Silencing of CENPF decreased mitochondrial oxidative phosphorylation. The mitochondrial biogenetic activity was determined for CENPF^KO and Ctrl in real-time using the Seahorse extracellular flux analyzer. (a) The oxygen consumption rate (OCR) curves in CENPF^KO cells and Ctrl treated with oligomycin, FCCP and rotenone/antimycin A (n = 6 independent experiments). The OCR curves were determined by using a Seahorse XF24 Analyzer. (b) Basal and maximum respiratory capacity normalized to the cell numbers in CENPF^KO cells and Ctrl. (c) Extracellular acidification rate (ECAR) was determined. (d) An energy map showing difference between CENPF^KO cells and Ctrl. (e) Western blot analysis was performed to measure the levels of TAM20 and OXPHOS proteins. (f) Western blot data showing phospho-PKM2 (Y¹⁰⁵) and PKM2 expression in CENPF^KO cells and Ctrl in presence or absence of pioglitazone. Both cells were treated with 10μM pioglitazone for 24 h before sample preparation. The graphs shown here are representative examples of three independent experiments. The data represent the mean ± standard deviation. A two-tailed Student’s t-test was used to calculate statistical significance.