Figure 2.
Phosphorylation of Fbxo6 is dynamically changing in cell cycle. (a) HeLa cells stably transfected with flag-Fbxo6 (HeLaflag-Fbxo6) were treated with thymidine (Thy) (2.5 mM) for 24 hours followed by nocodazole (200 ng/ml) treatment for the various times as indicated. DNA content of the treated cells was analyzed by flow cytometry (Asy for asynchronized HeLaflag-Fbxo6cells). (b)The indicated proteins were examined by western blot. (c) HeLaflag-Fbxo6 cells were treated with nocodazole (Noc, 200 ng/ml) for 16 hours and then released for the various times as indicated. DNA content of the treated cells was analyzed by flow cytometry. (d) The indicated proteins were examined by western blot.