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. 2018 Dec 18;17(24):2779–2789. doi: 10.1080/15384101.2018.1557488

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Figure 3. — Overexpression of Fbxo6 induces premature sister-chromatid separation. (a) The proliferation rate of HeLa^con and HeLa^flag-Fbxo6 cells for 5 days were analyzed by CCK8 assay. (b) DNA content of HeLa^con and HeLa^flag-Fbxo6 cells for 3 days was analyzed by flow cytometry. (c) HeLa^con and HeLa^flag-Fbxo6 cells were treated with nocodazole (200 ng/ml) for 16 hours. Mitotic chromosome spreads were prepared and stained by Wright-Giemsa dye. Arrows indicate prematurely separated sister chromatids. Representative images were shown. The percentages of cells with premature sister-chromatid separation were analyzed. P = 0.0004 vs, control group.