Figure 4.
Overexpression of Fbxo6 accelerates the exit from mitosis in nocodazole-released cells. HeLacon and HeLaflag-Fbxo6 cells were treated with nocodazole (200 ng/ml) and then released for the various times as indicated. (a) DNA content of the treated cells was analyzed by flow cytometry. (b) Cells treated in (a) were stained by Wright-Giemsa dye. Representative images were shown. (c) Percentages of cells with binuclear and multi-lobed nuclei, as well as dead cells were counted from cells treated in (a).