Figure 5.
Fbxo6 interacts with spindle check point proteins. (a) Lysates from HeLacon and HeLaflag-Fbxo6 cells stably expressing flag-con or flag-Fbxo6 were immunoprecipitated with anti-FLAG M2 affinity gel. Bound proteins were eluted with FLAG peptide and subjected to Western blot with BubR1, Mad2, Bub3, Cullin1 and flag antibodies. (b) HeLaflag-Fbxo6 cells were stained with Mad2 or BubR1 (Red), Fbxo6 (Green) and Dapi (Blue). (c) HeLa cells were transfected with flag-Fbxo6 at different dosages, and also arrested by thymidine (2.5 mM) at G1/S phase or by nocodazole (200 ng/ml) at G2/M phase. The indicated proteins were examined by western blot.