Figure 3.

PF384 induces G1/G0 cell cycle arrest and decreases cell viability in a non-caspase dependent manner in association with the induction of autophagy. a) BxPC-3 and MIA-Pa-Ca-2 cells were treated as indicated for 24 hours after which they were labelled with propidium iodide and the percentage of cells in each stage of the cell cycle determined by flow cytometry. b) Cells were treated as indicated for 6 hours after which cell viability was determined by MTT assay. Where z.VAD.fmk (100 μM) was used, cells were pre-incubated with this for 1 hour before the addition of other drugs. c) Cells were treated as indicated for 24 hours after which whole cell lysates were assessed for expression of LC3A/B and β-tubulin by western blotting. All data shown are the mean ± SEM for 3 independent experiments. Blots are representative of two biological repeats.