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MiR-135b negatively regulated Sp1. (a) The binding site between miR-135b and Sp1 3ʹUTR. (b) The luciferase activity of Sp1 3ʹUTR in HEK293 cells co-transfected with miR-135b mimics or miR-135b inhibitor. (c) The protein expression of Sp1 in C5.18 cells. Pre-NC was a control of miR-135b mimics; NC was a control of miR-135b inhibitor. *P < 0.05, vs Pre-NC or NC.
