Figure 10.
The expression of FN1 was regulated by NEAT1/miR-101-3p. (a) FN1 was overexpressed in 131I-resistant PTC cell lines determined by qRT-PCR. (b) TargetScan predicted the direct target relationship between miR-101-3p and FN1. (c) Luciferase reporter assay indicated that miR-101-3p directly targeted at FN1. (d) QRT-PCR indicated that upregulated of miR-101-3p inhibited the mRNA expression of FN1, and co-transfected with Si-NEAT1 increased the effect of inhibition. (e) Western blot supported that the co-transfection of miR-101-3p and Si-NEAT1 significantly decreased the protein expression of FN1, compared to control or transfected single. (f) Cell viability assay was detected by MTT, under 131I treatment for 96 h. FN1 attenuated the effect of RAI and Si-NEAT1 could reversed this resistance. (g) Apoptosis assay for res-TPC-1 cell line treated with 131I for 12 h was evaluated by flow cytometry. TPC-1 cells were treated with 1.0 mCi 131I and B-CPA cells were treated with 0.45 mCi 131I. The data were from one representative experiment of three identically performed and were expressed as mean±SD. ** P < 0.01, *** P < 0.001.