Table 1.
Primer sequences used for real-time PCR.
| Gene | Sequence(5ʹ-3ʹ) | |
|---|---|---|
| NEAT1 [Homo sapiens (human)] |
Forward primer | TGGCTAGCTCAGGGCTTCAG |
| Reverse primer | TCTCCTTGCC.AAGCTTCCTT | |
| MiR-101-3p [Homo sapiens (human)] |
Forward primer | UACAGUACUGUG AUAACUGAA |
| Reverse primer | CAGUUAUCACAGUACUGUAUU | |
| FN1 [Homo sapiens (human)] |
Forward primer | AGGAAGCCGAGGTTTTAACTG |
| Reverse primer | AGGAAGCCGAGGTTTTAACTG | |
| U6 [Homo sapiens (human)] |
Forward primer | GCTCGCTTCGGCAGCACAT |
| Reverse primer | AAAATATGGAACGCTTCACG | |
| GAPDH [Homo sapiens (human)] |
Forward primer | CGCTCTCTGCTCCTCCTGTTC |
| Reverse primer | ATCCGTTGACTCCGACCTTCAC | |
|
Neat1 [Mus musculus (house mouse)] |
Forward primer | AGGTCGGTGTGAACGGATTTG |
| Reverse primer | AGGTCGGTGTGAACGGATTTG | |
|
WT Braf [Mus musculus (house mouse)] |
Forward primer | GCCCAGGCTCTTTATGAGAA |
| Reverse primer | AGTCAATCATCCACAGAGACCT | |
|
Lox-BrafV600E [Mus musculus (house mouse)] |
Forward primer | GCCCAGGCTCTTTATGAGAA |
| Reverse primer | AGTCAATCATCCACAGAGACCT | |
|
Gapdh [Mus musculus (house mouse)] |
Forward primer | AGGTCGGTGTGAACGGATTTG |
| Reverse primer | GGGGTCGTTGATGGCAACA |