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. 2018 Dec 31;22(4):39–54. doi: 10.20463/jenb.2018.0029

Table 1. Oligonucleotides used for Real-time RT-PCR in this study.

Target 5’ – 3’ Sequence GenBank Accession Number
Leptin Sense CCAAAACCCTCATCAAGACC NM_008493
Antisense GTCCAACTGTTGAAGAATGTCCC
UCP2 Sense CCGCATTGGCCTCTACGACTCT NM_011671
Antisense CCCCGAAGGCAGAAGTGAAGTG
Adiponectin Sense CCCAAGGGAACTTGTGCAGGTTGGATG NM_009605.4
Antisense GTTGGTATCATGGTAGAGAAGAAAGCC
C/EBPα Sense TGGACAAGAACAGCAACGAGTAC NM_001287523.1
Antisense CGGTCATTGTCACTGGTCAACT
C/EBPβ Sense AAGCTGAGCGACGAGTACAAGA NM_001287739.1
Antisense GTCAGCTCCAGCACCTTGTG
SREBP1c Sense AGCCTGGCCATCTGTGAGAA XM_006532714.2
Antisense CAGACTGGTACGGGCCACAA
ACC1 Sense GCCATTGGTATTGGGGCTTAC NM_133360.2
Antisense CCCGACCAAGGACTTTGTTG
AMPKα1 Sense AAGCCGACCCAATGACATCA XM_011245321.1
Antisense CTTCCTTCGTACACGCAAAT
AMPKα2 Sense GATGATGAGGTGGTGGA NM_178143.2
Antisense GCCGAGGACAAAGTGC
GAPDH Sense CATCTTCCAGGAGCGAGACC NM_008084
Antisense TCCACCACCCTGTTGCTGTA

RT-PCR = reverse transcription polymerase chain reaction

UCP = Mitochondrial uncoupling protein

C/EBP = CCAAT-enhancer-binding protein

SREBP = Sterol regulatory element-binding protein

ACC1 = Acetyl-CoA carboxylase 1

AMPK = 5' adenosine monophosphate-activated protein kinase

GAPDH = Glyceraldehyde 3-phosphate dehydrogenase