Table 1. Oligonucleotides used for Real-time RT-PCR in this study.
Target | 5’ – 3’ | Sequence | GenBank Accession Number |
---|---|---|---|
Leptin | Sense | CCAAAACCCTCATCAAGACC | NM_008493 |
Antisense | GTCCAACTGTTGAAGAATGTCCC | ||
UCP2 | Sense | CCGCATTGGCCTCTACGACTCT | NM_011671 |
Antisense | CCCCGAAGGCAGAAGTGAAGTG | ||
Adiponectin | Sense | CCCAAGGGAACTTGTGCAGGTTGGATG | NM_009605.4 |
Antisense | GTTGGTATCATGGTAGAGAAGAAAGCC | ||
C/EBPα | Sense | TGGACAAGAACAGCAACGAGTAC | NM_001287523.1 |
Antisense | CGGTCATTGTCACTGGTCAACT | ||
C/EBPβ | Sense | AAGCTGAGCGACGAGTACAAGA | NM_001287739.1 |
Antisense | GTCAGCTCCAGCACCTTGTG | ||
SREBP1c | Sense | AGCCTGGCCATCTGTGAGAA | XM_006532714.2 |
Antisense | CAGACTGGTACGGGCCACAA | ||
ACC1 | Sense | GCCATTGGTATTGGGGCTTAC | NM_133360.2 |
Antisense | CCCGACCAAGGACTTTGTTG | ||
AMPKα1 | Sense | AAGCCGACCCAATGACATCA | XM_011245321.1 |
Antisense | CTTCCTTCGTACACGCAAAT | ||
AMPKα2 | Sense | GATGATGAGGTGGTGGA | NM_178143.2 |
Antisense | GCCGAGGACAAAGTGC | ||
GAPDH | Sense | CATCTTCCAGGAGCGAGACC | NM_008084 |
Antisense | TCCACCACCCTGTTGCTGTA |
RT-PCR = reverse transcription polymerase chain reaction
UCP = Mitochondrial uncoupling protein
C/EBP = CCAAT-enhancer-binding protein
SREBP = Sterol regulatory element-binding protein
ACC1 = Acetyl-CoA carboxylase 1
AMPK = 5' adenosine monophosphate-activated protein kinase
GAPDH = Glyceraldehyde 3-phosphate dehydrogenase