Figure 3.

Average Combined Chemical Shift Deviation (CCSD) precision plots of required experiments at 37 °C. (a) CCSD of ¹H,¹⁵N D1A/E2, E2B, and E2C spectra; (b) CCSD of D2A/D2C and D2B/D2D/D2E ¹H,¹³C spectra. All ¹H,¹⁵N spectra and ¹H,¹³C D2A/D2C spectra were acquired with uniform sampling, while ¹H,¹³C D2B/D2D/D2E spectra were 50% non-uniformly sampled. The number above each bar represents the total number of spectra included in the analysis for each respective experimental type. For ¹H,¹⁵N CCSD plot, E2 experiments were included since the only parameter change from D1A was a smaller ¹⁵N spectral width. Errors bars represent 95% confidence intervals of SEM. For detailed breakdown of experimental codes, see Table S10.