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Fragment ions and sequence map of the −58 Da proteoform of Fc/2. Top, representative fragment ions. Bottom, Fc/2 subunit sequence map showing bond cleavages, glycosylation site, loss of C-terminal glycine, and C-terminal proline amidation. The glycosylation site Asn61 and amidated Pro209 are highlighted in red. The amino acid number here is based on the Fc/2 subunit sequence.
