Figure 4.

Extended specificity and functional cytotoxicity testing of final TCR candidates arising from the c672 and c728 parental TCRs.

(A) Extended tumor cell line and primary cell screen of five candidate TCRs selected on the basis of initial characterization (see Figure 3). Blue points represent numbers of IFN-γ spot forming units (SFU) counted in triplicate wells in two donors, for TCR-transduced T-cells; grey points represent the responses of non-transduced T-cells (consistently < 50 SFU). MAGE-A10⁻ cell lines did not induce T-cell responses above the background level. (B) Potency testing of two final candidate TCRs c756 and c796 using a real-time cytotoxicity assay. Functional cytotoxicity was confirmed, and the magnitude and kinetics of the killing of MAGE-A10⁺ HLA-A*0201-transduced SK-Mel-28 cells were similar.