Figure 6.

Further analysis of peptide X-scan data confirming the superior specificity of the c796 MAGE-A10-specific TCR over c756.

(A) An illustration of the superior specificity of TCR c796 over c756, at any response cut-off value (defined as the fraction of the response to the index peptide at its EC₉₀ concentration (10^−7.5 M) at which a substitution is considered to be tolerated by the TCR). Numbers of unique human peptide matches, which conform to the consensus motif based on peptide X-scan data, are plotted against the response cut-off value, with solid lines representing the median number of matches derived from data from triplicate wells, and shaded areas representing the range between the lowest and highest numbers of matches from these wells. TCR c796 is shown in red, c756 in blue. (B) Motifs derived for TCR c796 identify fewer human nonamers than those for c756, at any value of p(Match) (defined as the naïve probability of identifying matching sequences within the proteome). Points represent numbers of unique human peptide matches to which the two final TCR candidates may show cross-reactivity, plotted against p(Match) values; c796 is shown as red triangles, and c756 as blue circles.