Table 2.

Glycopeptide mapping of MICA GEM variants.

WT	R202N	E215N	N234 (I236T)	G243N	H248N	R279N	N298.G299. S300	Chymotrypsin and/or GluC Peptide(s)
	22%							GSnRTVPPMVnVTRSE/GSnRTVPPMVNVTRSE
		98%						GSTVPPMVnVTRSnASE/GSTVPPMV[nVTRSn]ASE*
			97%					YPRnITL
				91%				nVSLSHD
					99%			RQDGVSLSnDTQQW
						92%		QnFTCYME
							> 99%	HSGnHSTHPVPSnGSGNSRAQVTD/ HSGNHSTHPVPSnGSGNSRAQVTD
0%	< 1%	< 1%	3%	4%	1%	0%	< 1%	GLHnHHTTKSFSRTPG#

*Doubly deamidated peptide identified (N210 & N215).

^#N328 is a non-N-linked glycosylated peptide control that shows false positive error rates of 0–4%.