Table 5.

Brief details of binding titrations contributing to the study.

Lab Code	Assay Platform	Assay description	Readout	Specific for infliximab
1	FRET	Europium labelled infliximab and Cy5 labelled TNF form fluorescent complex which is competitively inhibited by unlabelled infliximab	Resonance energy transfer	Yes
2	SPR	Infliximab titrated onto chip coated with anti-Fc and fixed concentration of TNF passed over	Response units at binding saturation	N/A
8, 18	ELISA	Plates coated with TNF, infliximab captured and detected with anti-Kappa chain-HRP and TMB substrate.	Absorbance 450nm	No
22, 24, 27	ELISA	Plates coated with anti-TNF, TNF captured, infliximab captured by bound TNF and detected by anti-infliximab-HRP and TMB substrate	Absorbance 450nm	Yes
23	ELISA	Plates coated with TNF, infliximab captured and detected with anti-IgG -HRP and TMB substrate	Absorbance 450nm	No