Figure 3.

Identification of lead Fabs after affinity maturation.

a) Dual luciferase assay measuring augmented inhibition of affinity matured Fabs relative to Fab 5775 (statistical significance compared to Fab 5775 are indicated by * p < 0.05, ** p < 0.01, *** p < 0.001). b) The IC₅₀ values for lead Fabs was determined in dual luciferase assays over a range of doses (1000–0.01 nM). c) The IC₅₀ values are provided for each Fab based on values obtained from 3 experiments. d) The effects of affinity matured Fabs on TGFβ signaling relative to Fab 5775 and SB431542 (SB) was analyzed by IB with pSMAD2/3 and pan SMAD2/3 antibodies in SKOV3 cells (all inhibitors used at 200 nM) and ID8/Trp53^−/- cells (all inhibitors used at 1 µM).