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Analysis of CpG methylation status of ICs and PD-L1 in circulation of HD, PBC and CRC patients. Representative plots show the promoter CpG methylation status in PD-1 (a), CTLA-4 (b), TIM-3 (c), LAG-3 (d), TIGIT (e) and PD-L1 (f) in the circulation of 10 HD, 8 PBC and 12 CRC patients as analyzed by bisulfite sequencing of the gDNA. Methylation status of individual CpG motifs is shown by white (demethylation) or grey (methylation) colors.
