. 2018 Oct 19;11(1):129–144. doi: 10.1080/19420862.2018.1530920

Table 2.

Disulfide linkage of SB5 and the reference product.

Type of Disulfide Bond		Cysteine site	Expected Mass (Da)	Identified m/z (Charge state)	Experimentally Detected m/z
Type of Disulfide Bond		Cysteine site	Expected Mass (Da)	Identified m/z (Charge state)	SB5	EU/US reference products
Heavy chain (Intra)	H^a:T^c3-H:T9	Cys₂₂-Cys₉₆	3406.47	682.30 (+ 5)	682.30	682.30
	H:T12-H:T13	Cys₁₄₈-Cys₂₀₄	7916.92	1320.49 (+ 6)	1320.49	1320.49
	H:T20-H:T26	Cys₂₆₅-Cys₃₂₅	2328.10	777.04 (+ 3)	777.04	777.04
	H:T34-H:T39	Cys₃₇₁-Cys₄₂₉	3844.82	641.81 (+ 6)	641.81	641.81
Light chain (Intra)	L^b:T2-L:T7	Cys₂₃-Cys₈₈	3818.78	764.76 (+ 5)	764.78	764.78
Light chain (Intra)	L:T13-L:T20	Cys₁₃₄-Cys₁₉₄	3555.75	712.16 (+ 5)	712.16	712.16
Inter chain	H:T17-L:T22	Cys₂₂₄-Cys₂₁₄	756.24	757.25 (+ 1)	757.25	757.25
Inter chain	H:T18 = H:T18	Cys₂₃₀-Cys₂₃₀ = Cys₂₃₃-Cys₂₃₃	5454.78	780.26 (+ 7)	780.26	780.26

^aH: Heavy chain, ^bL: Light chain, ^cT: Tryptic peptide