Figure 2.

Specificity analyses by flow cytometry using transiently-transfected HEK-293 cells.

Analyses of binding specificity were performed on HEK-293 cells transiently transfected with plasmids encoding either (A) human PD1, human VEGFR2, (B) human FZD5, human ULBP2. All plots show the target of interest transfected (grey line) versus ZS green marker-only transfected cells (black line). Transfected cells were stained using Mab005-IgG1, SHR-1210-IgG1, Pembrolizumab IgG1 null analog, and isotype IgG1. Each antibody was used in repeat staining at 5 μg/ml. These analyses confirmed that all antibodies (other than the isotype control IgG1) exhibited binding to PD1, but no antibody exhibited measurable signal on ZS-green transfected cells. Both Mab005-IgG1 and SHR-1210-IgG1 also exhibited strong binding to all targets, while the isotype IgG1 and Pembrolizumab analog did not.