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. 2018 Dec 12;11(1):26–44. doi: 10.1080/19420862.2018.1550321

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 10. — Off-target binding analysis ELISA assay.

SHR-1210-IgG1, Mab005-IgG1, library-derived clones and designer clones in human IgG1null format were titrated (in μg/ml) in a direct binding ELISA against human PD1 (A) and cyno PD1 (B), human VEGFR2 (C) and rhesus VEGFR2 (D), human FZD5 (E) and BSA (F) proteins. These analyses confirmed that all anti-PD1 antibodies exhibited binding to PD1, but only Mab005-IgG1 and SHR-1210-IgG1 exhibited measurable off-target binding to VEGFR2 and FZD5 proteins.