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. 2018 Oct 10;8(2):e1527650. doi: 10.1080/2162402X.2018.1527650

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Figure 4. — MBL deficiency is associated with an increase of activated HSCs.

(a) Analysis of activated HSCs (α-SMA⁺ immunoreactive area, IRA) in tumor tissue of tumor-bearing WT and MBL^–/– mice. Scale bars, 50 μm. α-SMA (b) and its mRNA expression (c) in liver tissue were analyzed by immunoblot assay and RT-PCR, respectively. (d) Immunofluorescence analysis of co-localization of MBL (green) andα-SMA (red) in situ in tumor tissues of WT mice and HCC patients. Scale bars, 25 μm. Quiescent HSCs (qHSCs) were separated from the normal WT mouse liver as described in Materials and Methods. Activated HSCs (aHSCs) were obtained by culture qHSCs with Hepal-6-CM. MBL (e) and its mRNA expression (f) in qHSCs and aHSCs were analyzed by immunoblotting and quantitative RT-PCR, respectively. Hepatocytes served as a positive control. The data representative of more than three independent experiments with similar results are shown.