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. 2018 Oct 10;8(2):e1527650. doi: 10.1080/2162402X.2018.1527650

Figure 8.

Figure 8.

AAV-mediated restoration of hepatic MBL abrogated MBL deficiency-promoted tumor growth.

The following parameters were measured in liver tumor tissues of WT and MBL–/– mice in the murine HCC model established as described in Figure 1. pAAV-con and pAAV-MBL vectors were administered 3 weeks before hepal-6 cell injection. (a) Liver tumor number, maximal tumor size and liver index were monitored. (b) The frequency of α-SMA+ activated HSCs in liver sections was evaluated by immunohistochemistry. (c) α-SMA and COX-2 protein expression and (d) PGE2 production in the liver were determined by immunoblotting and ELISA respectively. (e) MDSCs (Gr-1+CD11b+), (f) G-MDSCs (CD11b+Ly6ClowLy6G+) and M-MDSCs (CD11b+Ly6ChighLy6G−) frequency in liver tumor tissue. The percentages represent the cell frequency in CD45+ cells. Data are presented as means ± SEM (horizontal lines). NS, not significant. *, P < 0.05; **, P < 0.01. The data representative of at least three independent experiments with similar results are shown.