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. 2018 Oct 2;11(1):166–177. doi: 10.1080/19420862.2018.1524664

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Figure 2. — Affinity maturation and framework replacement of scFv ATROSAB (scFv IZI06.1) results in improved activity and stability.

Binding of the parental scFv IZI06.1, the intermediate scFvs IG11 and T12B and the final version scFv 13.7, after affinity maturation and framework replacement was analyzed in ELISA (a, mean ± SD, n = 2). Inhibition of TNF-induced (0.1 nM TNF) IL-8 release from HT1080 cells (b) was analyzed for all scFvs (displayed are mean ±SD, n = 3). Thermal stability of scFv IZI06.1(c), scFv T12B (d) and scFv 13.7 (e) was analyzed using dynamic light scattering. T_m was determined upon visual interpretation of the displayed data points.