Figure 4.

Bioactivity of 13.7 antibodies.

The potential of IgG13.7 and Fab13.7 to stimulate TNFR1 on the surface of HT1080 and Kym-1 cells was analyzed by detection of IL-8 (a) release as well as by the induction of cell death (b). The inhibitory activity of Fab13.7 in the respective assays was analyzed (c and d) with respect to receptor stimulation by 0.1 nM TNF (c) or 0.01 nM TNF (d). ATROSAB and FabATR served as controls. Stimulatory experiments were performed twice (a and b, n = 2, mean ±SD), inhibitory experiments were performed three times (c and d, n = 3, mean ±SD).