Table 3.

Affinity determination of IgG13.7 and Fab13.7.

	ATROSAB*	IgG 13.7*	Fab ATR	Fab 13.7
Bmax1 (Hz)	16.8		6.4	8.9
Bmax2 (Hz)	10.7	32.7	-	-
kon1 (M−1s−1)	2.8 × 105	-	5.0 × 105	4.7 × 105
koff1 (s−1)	2.2 × 10−2	-	1.5 × 10−2	7.3 × 10−4
KD1 (nM)	78	-	30	1.6
kon2 (M−1s−1)	1.1 × 106	6.6 × 105	-	-
koff2 (s−1)	4.1 × 10−4	5.4 × 10−4	-	-
KD2 (nM)	0.38	0.68	-	-

*apparent affinities, determined by measuring bivalent IgG binding to bivalent human TNFR1-Fc; 1: monovalent interaction; 2: bivalent interaction.