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. 2019 Jan 23;14(1):e0209748. doi: 10.1371/journal.pone.0209748

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© 2019 Wang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Immunoblot analysis of cell lines treated with various concentrations of GLB. Carbamazepine at 30 μM was used as a positive control. After 48h incubation of GLB or CBZ, cells were harvested and separated into soluble and insoluble fractions for western blotting with anti-AT. GAPDH was used as a loading control for the soluble fraction and to validate the separation of soluble and insoluble fractions. Gel Code blue staining was used a loading control for the insoluble fraction. The relative densitometric values for ATZ level in experimental versus control conditions are shown at the top to demonstrate the magnitude of the effect.