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. 2019 Jan 23;14(1):e0209748. doi: 10.1371/journal.pone.0209748

Fig 6. Effect of GLB analogs on ATZ levels and insulin secretion in mammalian cell line models.

Fig 6

(A) The structures of GLB and GLB analogs. (B) Western blotting analysis of the effect of G2, G3 and G4 on ATZ levels in HTO/Z cell line. GAPDH was included as a loading control. GLB at 40 μM was used as positive control; DMSO was used as negative control. (C) Insulin levels in Min-6 pancreatic β-cell line. The cells were incubated with GLB, G2, G3 and G4 in the indicated doses. Low and high glucose conditions were used as negative and positive controls (Mean ± SD, n = 3). The results show that insulin secretion is significantly increased by GLB at doses as low as 1 nM (p<0.0001) but not affected by analogs G2 (p = 0.8483), G3 (p = 0.9222) or G4 (p = 0.7886).