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. 2019 Jan 23;14(1):e0211188. doi: 10.1371/journal.pone.0211188

Fig 2. Effect of REAC treatment on the expression of pluripotency genes in hUiPSCs supplemented with defined medium.

Fig 2

Cells cultured in cardiogenic conditioned medium (Medium C) were exposed for 72 h, in the absence (grey bars) or presence (red bars) of REAC and then cultured in the presence of medium C alone until day 14th. The amount of OCT4(A), SOX3/4 (B), NANOG (C) and cMyc (D) mRNA from untreated (grey bars) or REAC-treated (red bars) cells was normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and was plotted as fold of change relative to the mRNA expression in hUiPSCs at day 0. Data shown mean±S.D; n = 4 (P<0.05 **), (P<0.001* **).