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. 2019 Jan 23;14(1):e0211188. doi: 10.1371/journal.pone.0211188

Fig 4. Effect of REAC treatment on the expression of early cardiac-specific marker genes in hUiPSCs supplemented with defined medium.

Fig 4

Cells cultured in cardiogenic conditioned medium (Medium C) were exposed for 72 hours, in the absence (grey bars) or presence (red bars) of REAC, and then cultured in the presence of medium C alone until day 14. The amount of ACTN2 (A), αMHC (B), and cTnT (C) mRNA from untreated (grey bars) or REAC-treated (red bars) cells was normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and was plotted as fold of change relative to the mRNA expression of hUiPSCs at day 0. Data shown mean±S.D; n = 4 (P<0.05 **), (P<0.001* **).