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. 2019 Jan 23;14(1):e0211188. doi: 10.1371/journal.pone.0211188

Fig 6. Effect of REAC treatment on the expression of cardiogenic markers in hUiPSCs after 14 days.

Fig 6

Total lysates were isolated from hUiPSCs committed by Cardiogenic medium (Medium C) in the absence or presence of REAC treatment for 72 hours. Samples were analyzed by Western blot, using antisera against α-Sarcomeric actinin (α-Sarc), cardiac troponin T (cTNT) and GAPDH (A). The sizes of the bands were determined using prestained marker proteins. The data presented are representative of five separate experiments. Two different membranes were incubated with anti-mouse (cTNT, GAPDH) or anti-rabbit (α-Sarc), densitometry analysis for α-Sarc (B) and cTnT (C) were performed with ImageJ.