. 2019 Jan 23;14(1):e0211188. doi: 10.1371/journal.pone.0211188

Table 1. Primers used in this work.

PRIMER NAME	REVERSE	FORWARD
ACTN2	`ACCAGTTTCACCCCTTTGCT`	`CGCCATGAACCAGATAGAGCC`
GATA4	`CTCAGATCCTTAGGTGCTAGA`	`TCCTCTTGCCTGGTAATGACTCC`
hMEF2C	`AGTGAGCTGACAGGGTTGCT`	`GCCCTGAGTCTGAGGACAAG`
hMHCα	`TTTGATGCGCCCGAACTCTT`	`GAGGAAATGAGGGACGAGAGG`
nkx2.5	`TAATCGCCGCCACAAACTCTCC`	`TATAACGCCTACCCCCGCCTAT`
TBX5	`GTGGGGAGCCATGGTTGGCC`	`CAGAGTCGGCACAGCGGCAA`
cTnT	`CGTCTCTCGATCCTGTCTTTG`	`CATGGAGAAGGACCTGAATGA`
GAPDH	`GACAAGCTTCCCGTTCTCAG`	`GAGTCAACGGATTTGGTCGT`

PCR products were confirmed by melting curve analysis and electrophoresis. All measurements were done as technical quadruplicate of biological triplicates. Biological replicates were obtained from independent differentiation setup. Relative expression was determined using “2^ΔΔCt method” with glyceraldehyde 3-phospate dehydrogenase (GAPDH) as normalizing expression levels.